Quantitative profiling of posttranslational modifications of pathological tau via sarkosyl fractionation and mass spectrometry
Kathrin Wenger, Arthur Viode, Mukesh Kumar, Hanno Steen, Judith A. Steen
Published: 2024-01-30 DOI: 10.1038/s41596-023-00939-z
Mass Spectrometry
Posttranslational Modifications
Tau Protein
Sarkosyl Fractionation
Quantitative Proteomics
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Supplementary information
Supplementary Information
Supplementary Fig. 1.
Supplementary Table 1
Transition list including monitored precursors and fragments of the FLEXITau assay.
Supplementary Data 1
Sequence of FLEXITau expression vector and the corresponding protein product.
Supplementary Data 2
Skyline library for FLEXITau data evaluation containing all monitored precursors and fragments of the FLEXITau assay.
Supplementary Data 3
Example for evaluating FLEXITau data, including the calculation of absolute total tau amount and the relative modification extent.
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