10x Protocols: Visium Fresh Frozen Library Construction -- University of Minnesota TMCs (CG000239 Rev F)
Laura J Niedernhofer, David A Bernlohr, 10X Genomics
Visium
Fresh Frozen
Library construction
10x Genomics
UMN
UMinnesota
University of Minnesota
OCT
Spatial
Abstract
Protocols from 10x Genomics for Visium Spatial Gene Expression on Fresh Frozen / OCT samples.
Completed without CytAssist component.
Version of protocol used:
10x protocol CG000239, Revision F -- Library Construction
Additional protocols added for guidance:
10x protocol for imaging guidelines (CG000241, Rev D)
10x protocol for tissue preparation guidelines (CG000240, Rev D)
Note: These protocols may not be the current version offered by 10x Genomics but were used to produce the specific datasets connected to them. Please review the 10x Genomics support website for the most recent versions of the protocols prior to starting your experiment.
Steps
10x protocol CG000239, Rev F (Library construction):
10x FF Visium Spatial Gene Expression User Guide.pdf
Additional Protocols/Guidelines
10x FF Visium Imaging Guidelines .pdf CG000241, Rev D
10x FF Visium Spatial Tissue Preparation Guide.pdf CG000240, Rev D
http://www.10xgenomics.com/support/spatial-gene-expression-fresh-frozen
